Human CLIA Kit for Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2)
检测样本类型:
Tissue homogenates, cell lysates and other biological fluids
靶点:
Nucleotide Binding Oligomerization Domain Containing Protein 2
Synoyms:
NOD2;Nucleotide Binding Oligomerization Domain Containing Protein 2; CD; ACUG; BLAU; CARD15; CLR16.3; IBD1; NLRC2; NOD2B; PSORAS1; Caspase recruitment domain-containing protein 15; Inflammatory bowel disease protein 1
检测方法:
双抗夹心(定量分析)
灵敏度:
less than 17.4pg/mL
检测时间:
2 hours 40 minutes
特异性:
This assay has high sensitivity and excellent specificity for detection of Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). No significant cross-reactivity or interference between Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) and analogues was observed.
精密度:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%.
稳定性:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
检测原理:
The microplate provided in this kit has been pre-coated with an antibody specific to Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Nucleotide Binding Oligomerization Domain Containing Protein 2 (NOD2) level in the sample or standard.;
实验流程:
1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37℃;
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37℃;
4. Aspirate and wash 3 times;
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37℃;
6. Aspirate and wash 5 times;
7. Add 100ul Substrate Solution. Incubate 10 minutes at 37℃;
8. Read RLU value immediately.