This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A1 (APOA1). No significant cross-reactivity or interference between Apolipoprotein A1 (APOA1) and analogues was observed.
精密度:
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A1 (APOA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A1 (APOA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%.
稳定性:
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
检测原理:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A1 (APOA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A1 (APOA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A1 (APOA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein A1 (APOA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
实验流程:
1. Prepare all reagents, samples and standards;
2. Add 100ul standard or sample to each well. Incubate 2 hours at 37℃;
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37℃;
4. Aspirate and wash 3 times;
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37℃;
6. Aspirate and wash 5 times;
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37℃;
8. Add 50ul Stop Solution. Read at 450nm immediately.