Polyclonal Antibody to Caspase 3 (CASP3)
PAA626Ra01
CASP3; Caspase 3; CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1
WB, IHC, ICC, IP
Rat, Human, Mouse, Porcine
Rabbit
产品名称: Polyclonal Antibody to Caspase 3 (CASP3)
靶点: CASP3
Synoyms: CASP3; Caspase 3; CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1
UniProt:

P55213

宿主来源: Rabbit
免疫原: RPA626Ra01-Recombinant Caspase 3 (CASP3)
反应种属: Rat, Human, Mouse, Porcine
应用: WB; IHC; ICC; IP.
预测分子量: Loading.....Please refer to the UniProt database
检出分子量: Loading.....Please contact us
使用方法: Western Blot(WB): 0.01-2µg/mL;
Immunohistochemistry(IHC): 5-20µg/mL;
Immunocytochemistry(ICC): 5-20µg/mL.
※Optimal working dilutions must be determined by end user.
克隆类型: Polyclonal
偶联: Unconjugated
浓度: 0.5mg/ml
储存溶液: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
储存条件: Store at 4℃ for frequent use.
Stored at -20℃ in a manual defrost freezer for one year without detectable loss of activity.
※Avoid repeated freeze-thaw cycles.
纯化方式: Affinity purification
Appearance: Liquid

Figure. Western Blot.
Sample: Mouse Lung lysate
Primary Ab: 0.1µg/ml Rabbit Anti-Rat CASP3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb19)

Figure. Western Blot.
Sample: lysates of Jurkat cell line treated with 1 uM staurosporine (STS) for the indicated times.
Primary Ab: 2µg/ml Rabbit Anti-Rat CASP3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb19)

Figure. Western Blot.
Sample: Lane1: Porcine Liver lysate; Lane2: Porcine Lung lysate; Lane3: Porcine Spleen lysate; Lane4: Jurkat cell lysate
Primary Ab: 0.1µg/ml Rabbit Anti-Rat CASP3 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb19)

Figure.FITC staining on IF.
Samples: Human U2OS cell;
Primary Ab: 20μg/ml Rabbit Anti-Rat CASP3 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb18)

Figure.DAB staining on IHC-P.
Samples: Rat Spleen Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat CASP3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: SAA544Rb19)

Figure. Western Blot.
Sample: Immunogen-Recombinant CASP3, Rat.

Figure.DAB staining on IHC-P.
Sample: Rat Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Rat CASP3 Antibody;
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)

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